For years, scientists have been concerned about chemicals in the environment, such as BPA, that mimic the estrogens found in the body. Until now, however, experimental limitations have prevented researchers from exploring what happens when - as in the real world - an individual is exposed to multiple estrogen-mimicking chemicals at the same time.
But now, University of Texas Medical Branch at Galveston researchers have used new techniques to study exposure to low doses of multiple xenoestrogens - and they've come to some disturbing conclusions.
Using cell cultures to test mixtures of three compounds known to affect estrogen signaling, - bisphenol A (BPA, found in plastic products), bisphenol S (a supposedly safer replacement for bisphenol A recently found to have similar effects) and nonylphenol (a common component of industrial detergents and surfactants) - the researchers determined that combinations of endocrine disruptors could have a dramatically greater effect than any one of them alone.
"We wanted to see how these persistent, ubiquitous contaminants affect estrogenic signaling when they're mixed together as they are in nature, so we set up a cell-culture system that allowed us to test their influence on signaling by estradiol, the estrogen found in adult, cycling women," said senior researcher Cheryl Watson. "What we found is that these things gang up on estradiol and thwart its response, which is not a good thing."
Watson tested different mixtures of estrogen-disrupting compounds using rat pituitary cells (master regulators of the animals' endocrine systems). The experiments measured the responses of key signaling pathways that lead to cell proliferation, the secretion of the pituitary hormone prolactin and the activation of proteins involved in apoptosis (programmed cell death), comparing the effects of estradiol alone with those of estradiol and mixtures of bisphenol A, bisphenol S and nonylphenol.
"These compounds work at very low concentrations - at the parts per trillion or parts per quadrillion level - and when you mix them together they affect estrogenic signaling differently and more dramatically than they do individually," Watson said. "We need to pay attention to this, because estrogens influence so many things in both males and females - reproduction, the immune system, metabolism, bone growth, all sorts of important biological functions."
Studies have detected measurable levels of bisphenol A and bisphenol S in the urine of more than 90 percent of Americans. According to Watson, modern humans are exposed to dozens of xenoestrogens more or less continually. "These things are all over the environment, and we need to know what they do so we can start figuring out what we need to change," Watson said. "They're probably disrupting and confusing hormones in people, and it's important to find a way to prevent that as soon as we can."
Discuss this article in our forum
Canada Labels BPA Baby Bottles "Toxic"
Plastic chemical BPA linked to PCOS
More Worrying Findings On Effects Of Common Chemical
Plastics chemical causing epigenetic fertility problems
Source: University of Texas Medical Branch at Galveston